Effect of different metals on protease activity in sunflower cotyledons
نویسندگان
چکیده
Financial support: Grants from the Universidad de Buenos Aires (UBA) and from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) (Argentina). Abbreviations: 2,4 DNPH: 2,4-dinitrophenylhydrazine AEBSF: 4-(2-aminoethyl) benzenesulfonyl fluoride cys: cysteine DAB: diaminobenzidine DNP: dinitrophenyl DTT: dithiothreitol HRP: horseradish peroxidase IAA: iodoacetamide PAGE: polyacrylamide gel electrophoresis PMSF: phenyl-methanesulfonyl fluoride PVP: polyvinylpyrrolidone ROS: reactive oxygen species SDS: sodium dodecyl sulfate protease activity, gelatinase profile and protein oxidation were examined in sunflower cotyledons. Our results indicate that this tissue has mainly acid proteases belonging to different classes. Cu over the control), they altered proteolysis in different ways. Pb, Al and Ni treatment decreased protease activity 22, 28 and 30% respect to control while Cd and Hg increased this activity in 23 and 27%. In Zn, Cu and Co treatments protease activity remained similar to control treatment. These results indicate that different proteases are involved in plant defence against metal toxicity. However, the identification of specific
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